phospho-creb (p-creb, ser133) (87g3) rabbit mab #9198 antibody  (Cell Signaling Technology Inc)

Journal: Journal of Nanobiotechnology

Article Title: Conversion of nanoscale topographical information of cluster-assembled zirconia surfaces into mechanotransductive events promotes neuronal differentiation

doi: 10.1186/s12951-016-0171-3

Figure Lengend Snippet: The interaction with the neuritogenesis-inducing surface has an impact on transcription factor dynamics relevant for neuronal differentiation. a The confocal images show the average stack projection of p-CREB stainings of cells in the indicated experimental conditions. The cells were fixed with 4 % PFA 60 min or 24 h after in the indicated conditions and stained for the nucleus (HOECHST), f-actin and p-CREB. The outer dashed lines represent the outlines of the cells obtained from the f-actin staining and the inner dashed line the nuclear area determined from the HOECHST staining. The graph (representing the global statistics of two independent experiments, n = 33–76 cells) summarizes the corresponding quantification performed with the help of ImageJ (see also “ ”). b The same experimental procedure and quantification (from three independent experiments, n: > 500 cells, >150 neurites) as in Additional file : Figure S2A–C, but with an inhibitor against JNK/c-jun (SP600125 10 and 20 µM). Representative images of the conditions can be found in Additional file : Figure S3

Article Snippet: Antibodies or fluorescence reagents: 4B4 (Beckman Coulter) and K20 (Santa Cruz Biotechnology, Santa Cruz, USA, California) against β1 integrin, 87G3 antibody against p-CREB (Cell signaling, Danvers, USA, Massachusetts), hVin-1 antibody against vinculin (Sigma-Aldrich), HOECHST 33342 (Molecular Probes (Thermo Fischer Scientific), Waltham, USA, Massachusetts), TRITC-Phalloidin (Sigma-Aldrich).

Techniques: Staining